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GoldHi EndoFree Plasmid Maxi Kit

金牌超量无内毒素质粒大提试剂盒(100-300 ml)

¥488.00 ¥698.00

CW2104M

10 preps

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内毒素是质粒提取中常见的污染物,由于真核细胞对内毒素非常敏感,因此,如果质粒中含有内毒素会大大降低真核细胞转染效率。本试剂盒提供一种简单快捷高效提取无内毒素质粒的新方法,在碱裂解法裂解细胞的基础上,采用独特的硅基质膜吸附技术,高效专一的结合质粒DNA;同时采用特殊的缓冲液系统和去内毒素buffer,有效去除内毒素、基因组DNA、RNA、蛋白等杂质。每次可处理100-300 ml菌液,获得多至2 mg转染级质粒DNA,整个提取过程只需50分钟。本试剂盒所得质粒纯度高、提取量大,特别适用于细胞转染,同时也可用于DNA测序,PCR,体外转录,内切酶消化等实验。
保存条件 运输条件
RT(15-30℃) 常温

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